Laboratory for Olive Cultivation

  • Collection and conservation of olive genetic material for biodiversity protection and utilization in genetic improvement.
  • Identification, characterization, improvement and valorization of Greek and foreign cultivars according to their pedoclimatic requirements,  their tolerance in pests and diseases, their suitability for high density plantations, aiming at the improvement of fruit and olive oil yield and quality.
  • Modernization of orchard management practices.
  • Study of olive flowering biology and of pollination incompatibility.
  • Research on organic agriculture and integrated production systems.
  • Study of olive production systems on the environment and on the socio-economic aspects of rural areas.
  • Research on climate change - olive growing interaction.
  • Precision agriculture and remote sensing applications in olive qrowing.

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  • Adaptation of the main Greek and foreign cultivars in the environment of Crete.
  • Identification and characterization of 42 Greek and 63 foreign cultivars and their conservation in the national olive germplasm collection.
  • Detemination of appropriate planting distance, tree shape formation, pruning type and regeneration pruning for the main olive cultivars.
  • Assessment of suitability of several olive cultivars to mechanical harvesting and combination of chemical sprays with mechanized harvest machines.
  • Identification of best performing harvesting machines (in terms of fruit removal/time ratio as well as of the least shoot damages) for minimizing the harvesting cost.
  • Identification of appropriate cultivar combinations for improved fruit yield and reduced climate-induced damages risk.
  • Collection of experimental data for sustainable organic olive farming practices.
  • Collection of experimental data for viable application of integrated production system.
  • Studies on the impact of climate change (high temperature, low temperature, UV-B radiation, water salinity) on olive.

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Research Projects
  • Modelling solutions for improved and resilient management strategies for olive tree against future climate change OLIVEMIRACLE Program FACCEE JPI. 2016-2019.
  • Introduction of new olive crop management practices focused on climate change mitigation and adaptation – oLIVE-CLIMA (LIFE11 ENV/GR/942). 2012-2017.
  • Survey of the olive growing area Makri in the Regional Administration of Eastern Macedonia & Thrace for selection and morphological description of representative genotype. 2012-2017
  • Training olive sector's stakeholders in a remote region of Greece towards sustainable production, processing and marketing of olive products. International Olive Council (SUB-IOC-TEC 4/12). 2012.
  • Promoting sustainable production and consumption patterns: the example of olive oil– INFOIL (LIFE08 INF/GR/000581). 2010-2012.
  • Safeguard of hazelnut and almond genetic resource: from traditional uses to novel agroindustry opportunities-SAFENUT, 2007-2010.
  • Integrated System for Identification, Analysis and Promotion of Olive Oil. Regional Innovation Pole of Crete "i4Crete". Hellenic General Secretariat for Research and Technology. Code 11-ΠΠΚ06, Α8. GSRT. 2006-2008
  • Application of Organic Agriculture in Olive in the framework of Reg. 2080/05 (Action Βiv.2). ΕOP ELAIOURGIKI, MIC ΕPΕ-ICAP A.E.
  • Application of Integrated Production in Olive. In the framework of Reg. 2080/05 (Action Βii). ΕOP ELAIOURGIKI, MIC ΕPΕ-ICAP A.E.
  • Sustainable use of water resources and rural development in drought affected areas–IMAGE. Interreg III B Archimed, Measure 3.1: Protection, Planning of natural resources and Landscape, 2006-2008.
  • Mediterranean Initiatives-Development in Agriculture-M.I.D.A.. Interreg III B Archimed, Measure 1.2: Assistance for urban centres, rural areas and growth of cooperation between them, 2006-2008.
  • Effect of salinity stress on olive plantlets in Hydroponics cultivation and alleviation techniques, Transnational Collaboration, GSRT. 2007-2008.
  • "The future of olive plantation systems on sloping and mountainous land – Scenarios for production and natural resource conservation". OLIVERO. 2003-2006. Ε.U.

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  • Koubouris, G., Bouranis, D., Vogiatzis, E., Nejad, A.R., Giday, H., Tsaniklidis, G., Ligoxigakis, E.K., Blazakis, K., Kalaitzis, P., Fanourakis, D. (2018) Leaf area estimation by considering leaf dimensions in olive tree. Scientia Horticulturae, 240, pp. 440-445.
  • Bechara, E., Papafilippaki, A., Doupis, G., Sofo, A., Koubouris, G. (2018) Nutrient dynamics, soil properties and microbiological aspects in an irrigated olive orchard managed with five different management systems involving soil tillage, cover crops and compost. Journal of Water and Climate Change. DOI: 10.2166/wcc.2018.082.
  • Kavvadias, V., Papadopoulou, M., Vavoulidou, E., Theocharopoulos, S., Repas, S., Koubouris, G., Psarras, G., Kokkinos G. (2018) Effect of addition of organic materials and irrigation practices on soil quality in olive groves. Journal of Water and Climate Change. DOI: 10.2166/wcc.2018.090.
  • Farinelli, D. , Breton, C. , Koubouris, G. , Famiani, F. , Villemur, P. and Bervillé, A. (2018), Reply to Saumitou‐Laprade et al. (2017) “Controlling for genetic identity of varieties, pollen contamination and stigma receptivity is essential to characterize the self‐incompatibility system of Olea europaea L.”. (I.F. 5.67). Evol Appl.. doi:10.1111/eva.12633.
  • Koubouris, G., Psarras, G., Doupis, G. (2018) Potential for enrichment of soil with mineral nutrients by recycling olive pruning residues. Acta Horticulturae, 1199, pp. 297-301.
  • Arampatzis, G., Hatzigiannakis, E., Kourgialas, N., Psarras, G., Kinigopoulou, V., Panagopoulos, A., Koubouris, G. (2018) Seasonal variation and implications of soil water content in the cultivation of olive trees. Acta Horticulturae, 1199, pp. 339-343.
  • Sentouka, A., Sergentani, C., Markakis, E., Koubouris, G. (2018) Impact of fruit thinning on fruit properties of Olea europaea 'Kalamata' and 'Manzanilla'. Acta Horticulturae, 1199, pp. 375-378.
  • Victor Kavvadias, Maria Papadopoulou, Evangelia Vavoulidou, Sideris Theocharopoulos, Stella Malliaraki, Katerina Agelaki, Georgios Koubouris, Georgios Psarras, (2018) Chapter 10 - Effects of Carbon Inputs on Chemical and Microbial Properties of Soil in Irrigated and Rainfed Olive Groves, Editor(s): María Ángeles Muñoz, Raúl Zornoza, Soil Management and Climate Change, Academic Press, Pages 137-150, ISBN 9780128121283. .
  • Breton, C., Koubouris, G., Villemur, P., & Bervillé, A. J. (2017). 'Comment on Saumitou et al. (2017): Elucidation of the genetic architecture of self-incompatibility in olive: Evolutionary consequences and perspectives for orchard management'. Evolutionary Applications, doi:10.1111/eva.12494.
  • Kourgialas, N. N., Karatzas, G. P., & Koubouris, G. C. (2017). A GIS policy approach for assessing the effect of fertilizers on the quality of drinking and irrigation water and wellhead protection zones (Crete, Greece). Journal of Environmental Management, 189, 150-159. doi:10.1016/j.jenvman.2016.12.038.
  • Markakis, E.A., Kavroulakis, N., Ntougias, S., Koubouris, G.C., Sergentani, C.K., Ligoxigakis, E.K. (2017) Characterization of fungi associated with wood decay of tree species and grapevine in Greece. Plant Disease, 101 (11), pp. 1929-1940.
  • Koubouris, G.C., Kourgialas, N.N., Kavvadias, V., Digalaki, N., Psarras, G. (2017) Sustainable Agricultural Practices for Improving Soil Carbon and Nitrogen Content in Relation to Water Availability–An Adapted Approach to Mediterranean Olive Groves. Communications in Soil Science and Plant Analysis, 48 (22), pp. 2687-2700. doi: 10.5004/dwt.2017.21548.
  • Kourgialas, N. N., G., Doupis, G., Psarras, C., Sergentani, N., Digalaki, and G. C., Koubouris. (2017). Soil management and compost effects on salinity and seasonal water storage in a Mediterranean drought-affected olive tree area. Desalination and Water Treatment 1–7.
  • Aksehirli-Pakyurek, M., Koubouris, G. C., Petrakis, P. V., Hepaksoy, S., Metzidakis, I. T., Yalcinkaya, E., & Doulis, A. G. (2017). Cultivated and wild olives in Crete, Greece—Genetic diversity and relationships with major Turkish cultivars revealed by SSR markers. Plant Molecular Biology Reporter, 35(6), pp. 575-585. doi:10.1007/s11105-017-1046-y.
  • Kourgialas, N. N., Koubouris, G. C., Karatzas, G. P., & Metzidakis, I. (2016). Assessing water erosion in Mediterranean tree crops using GIS techniques and field measurements: The effect of climate change. Natural Hazards, 83, 65-81. doi:10.1007/s11069-016-2354-5.
  • Breton, CM., Farinelli, D., Koubouris, G., Bervillé, A. (2016) A model based on S-allele dominance relationships to explain pseudo self-fertility of varieties in the olive tree. Euphytica 210, 105–117.
  • Garantonakis, N., Varikou, K., Markakis, E., Birouraki, A., Sergentani, C., Psarras, G., Koubouris, G. (2016) Interaction between Bactrocera oleae (Diptera: Tephritidae) infestation and fruit mineral element content in Olea europaea (Lamiales: Oleaceae) cultivars of global interest. Applied Entomology and Zoology 51, 257-265
  • Kourgialas, N., Doupis, G., Papafilippaki, A., Psarras, G., Koubouris, G. (2016) Seasonal Variation of Soil Moisture in Irrigated Olive Trees. Procedia Engineering 162, pp. 471-475.
  • Koubouris, G. C., Tzortzakis, N., Kourgialas, N. N., Darioti, M., & Metzidakis, I. (2015). Growth, photosynthesis and pollen performance in saline water treated olive plants under high temperature. International Journal of Plant Biology 6(1), 28-32.
  • Koubouris, G.C., Kavroulakis, N., Metzidakis I.T., Vasilakakis, M.D., Sofo, A. (2015) Ultraviolet-B radiation or heat cause changes in photosynthesis, antioxidant enzyme activities and pollen performance in olive tree. Photosynthetica 53, 279-287.
  • Xanthopoulou, A., Ganopoulos, I., Koubouris, G., Tsaftaris, A., Sergendani, C., Kalivas, A., Madesis, P. (2014) Microsatellite high-resolution melting (SSR-HRM) analysis for genotyping and molecular characterization of an Olea europaea germplasm collection. Plant Genetic Resources: Characterization and Utilization, 1-5, doi:10.1017/S147926211400001X.
  • Koubouris, G.C., Breton, C., Metzidakis I.T., Vasilakakis M.D.. (2014) Self-incompatibility and pollination relationships for four Greek olive cultivars. Sci. Hortic. 176, 91-96.
  • Koubouris, G.C. Metzidakis I.T., Vasilakakis, M.D. (2012) Intraspecific variation in pollen viability, germination and ultrastructure of Olea europaea L.. African J. Biotechnol. 11, 13442-13446.
  • Koubouris, G.C. Metzidakis I.T., Vasilakakis, M.D. (2012) Flower Performance and Fruit Persistence in Organic "Kalamata" Olive. Acta Hortic. 949, 203-208.
  • Metzidakis I.T., Koubouris G.C., Kassidonis E., Sergendani C., Giannakaki A., Papathanasiou, G., Kosmas C., Moustakas N. (2012) Impact of soil management practices on physical and chemical properties of soils formed in marls, conglomerates or schists in sloping olive groves. Acta Hortic. 949, 371-378.
  • Koubouris, G.C., Metzidakis I.T., Vasilakakis, M.D. (2010) Phenological, morphological and functional indicators of genetic variability and their implication in the sexual reproductive system of Olea europaea L. (Oleaceae). Sci. Hortic. 123, 547–550.
  • Koubouris, G.C., Metzidakis I.T., Vasilakakis, M.D. (2010) Influence of cross pollination on the development of parthenocarpic -shotberries- olive (Olea europaea L.) fruits. Expl.Agric. 46, 67–76.
  • Koubouris, G.C. Metzidakis I.T., Vasilakakis, M.D. (2009) Impact of temperature on olive (Olea europaea L.) pollen performance in relation to relative humidity and genotype. Environ. Exp. Bot. 67, 209–214.
  • Gómez  J.A., Amato M., Celano G., Koubouris G. (2008) Organic olive orchards on sloping land: more than a specialty niche production system?. J. Env. Manag. 89, 99-109.
  • Metzidakis I.T., Martinez-Vilela, A., Castro Nieto, G., Basso, B. (2008) Intensive olive orchards on sloping land: requiring good water and pest management. J. Env. Manag. 89, 120-128.
  • Owen, C.A., Bita, E.C., Banilas, G., Hajjar, S.E., Sellianakis, V., Aksoy, U., Hepaksoy, S., Chamoun, R., Talhook, S.N., Metzidakis, I., Hatzopoulos, P., Kalaitzis, P. 2005. AFLP reveals structural details of genetic diversity within cultivated olive germplasm from the Eastern Mediterranean. Theor. Appl. Genet. 110: 1169-1176.
  • Terzopoulos, P., Kolano, B., Bebeli, P., Kaltsikis, P., Metzidakis I. (2005) Identification of Olea Europaea L. cultivars using intersimple sequence repeat markers. Sci. Hortic. 105, 45-51.
  • Nikoloudakis N., Metzidakis I., Banilas G., Gazis F., Hatzopoulos, P. (2003) Discrimination and genetic diversity among cultivated olives of Greece using RAPD markers. J. Amer. Soc. Hort. Sci. 128, 741-746.


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Dr. Georgios Koubouris

Researcher, Head of the Laboratory

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Tel: +30 28210-83434

Dr. Ioannis Metzidakis

Emeritus Researcher

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Tel: +30 28210-83434

Chrysa Sergendani

Technical Agronomist

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Tel: +30 28210-83435

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