Laboratory of Physiology & Plant Mineral Nutrition

  • Study the mineral nutrition of olive tree, citrus, subtropical crops and vegetables
  • Apply leaf and soil analyses as a tool for nutrient deficiency correction and sustainable fertilizing of crops
  • Study fruit tree physiology and response to abiotic stresses
  • Alternative and environmental friendly practices for fertilizing crops
  • Services to the local agricultural sector in the field of plant mineral nutrition


  • Application of best agricultural practices related to plant mineral nutrition
  • Surveys for recording crop nutritional status and adaptation of fertilizer application
  • Adaptation of the foliar application of fertilizers for the local conditions
  • Diagnosis and correction of the most common nutrient deficiencies for the local crops
  • Improvement of avocado yield potential by using different cultural practices (girdling, application of chemicals to improve fruit set, etc.)
  • Use of antagonism in nutrient uptake to minimize the negative effects of salinity
  • Use of olive mill wastewater to improve soil fertility.

nutr2  nutr6

Research Projects

Indicative list of recent Projects with participation of our Lab:

  • "Sustainable management of biotic and abiotic parameters in hydroponic culture of tomato and lettuce" (HYDROFLIES), INTERREG III, 2012-2014
  • "Introduction of new olive crop management practices focused on climate change mitigation and adaptation" (OLIVE-CLIMA), LIFE11, 2012-2017.
  • "Biotechnological recycle of olive mills washing water by microalgae" (ALGATEC) in the FP7-SME-2008-1 της Ε.Ε. (Grant agreement: 232331). (
  • "Innovation Center of Crete with emphasis on natural resources management". EEA Grants (2010-2012)
  • "Characterization and Management of Olive Mill Wastewater" Cooperation for R&T between Greece-Montenegro (2006 - 2008)
  • "Innovative irrigation techniques for improved water use efficiency and fruit quality" in Be-lateral Cooperation for R&T between Greece-Tunisia (contract GSRT-135e).
  • "Safe and high quality food production using low quality waters and improved irrigation systems and management" (SAFIR) in the FP6-2004-FOOD-3-B της Ε.Ε. (contract FOOD-CT-2005-023168). (
  • "Study of the effect of potassium on tomato growth, yield and distribution of mineral elements in the plant, under saline water irrigation conditions". Funded by the GSRT - Greek - Yugoslavian Cooperation for Research and Technology. (2002-2004).
  • "Providing guidelines for the sustainable use of fertilizers in olive orchards according to soil and leaf analyses and crop information". Funded by EOP ELEOURGIKI. (2004).

nutr4  nutr5


Indicative list of publications:

  • PSARRAS G., CHARTZOULAKIS K., KASAPAKIS I., KLOPMANN W. 2014. Effect of different irrigation techniques and water qualities on yield, fruit quality and health risks of tomato plants. Acta Hort. 1038: 601-608.
  • DOUPIS G., BERTAKI M., PSARRAS G., KASAPAKIS I., CHARTZOULAKIS K. 2013. Water relations, physiological behavior and antioxidant defence mechanism of olive plants subjected to different irrigation regimes. Sci. Hort., 153: 150-156.
  • GHRAB M., GARGOURI K., BENTAHER H., CHARTZOULAKIS K., AYADI M., BEN MIMOUN M., MASMOUDI M.M., BEN MECHLIA N., PSARRAS G. 2013. Water relations and yield of olive tree (cv. Chemlali) in response to partial root-zone drying (PRD) irrigation technique and salinity under arid climate. Agricultural Water Management, 123: 1-11.
  • FORSLUND A., ENSINK J.H.J, MARKUSSEN B., BATTILANI A., PSARRAS G., GOLA S., SANDEI L., FLETCHER T., DALSGAARD A. 2012. Escherichia coli contamination and health aspects of soil and tomatoes (Solanum lycopersicum L.) subsurface drip irrigated with on-site treated domestic wastewater. Water Research 46: 5917-5934.
  • PSARRAS G., KASAPAKIS I., STEFANOUDAKI E., PAPADAKIS I., CHARTZOULAKIS K. 2011. Effect of different irrigation regimes on olive tree (Olea europaea L., cv. Koroneiki) physiology, yield and fruit quality. Acta Hort. 888: 89-94.
  • CHARTZOULAKIS K., PSARRAS G., MOUTSOPOULOU M., STEFANOUDAKI E. 2010. Application of olive mill wastewater to a Cretan olive orchard: Effects on soil properties, plant performance and the environment. Agriculture Ecosystems & Environment, 138: 293-298.
  • JENSEN C.R., BATTILIANI A., PLAUBORG F., PSARRAS G., CHARTZOULAKIS K., JANOWIAK F., STIKIC R., JOVANOVIC Z., LI G., QI X., LIU F., JACOBSEN S.E., ANDERSEN M.N. 2010. Deficit irrigation based on drought tolerance and root signalling in potatoes and tomatoes. Agric. Water Manag. 98: 403-413.
  • MATON L., PSARRAS G., KASAPAKIS G., LORENZEN J.R., ANDERSEN M., BOESEND M., BAK S.N., CHARTZOULAKIS K., PEDERSEN S.M., KLOPPMANN W. 2010. Assessing the net benefits of using wastewater treated with a membrane bioreactor for irrigating vegetables in Crete. Agric. Water Manag. 98: 458-464.
  • PSARRAS G., BERTAKI M, CHARTZOULAKIS K., 2008. Response of greenhouse tomato to salt stress and K+ supplement. Plant Biosystems 142 (1): 149-153
  • CHARTZOULAKIS K., PSARRAS G., VEMMOS S., LOUPASSAKI M., BERTAKI M., 2006. Response of two olive cultivars to salt stress and potassium supplement. J. Plant Nutr. 29(11): 2063-2078.
  • CHARTZOULAKIS, K., PSARRAS, G. 2005. Impacts of global change on crop photosynthesis and production in Mediterranean: the case of Crete. Agriculture, Ecosystems and Environment. 106: 147-157.
  • LOUPASSAKI, Μ., CHARTZOULAKIS, Κ., DIGALAKI, Ν. and ANDROULAKIS, Ι., 2002. Effects of salt stress on concentation of nitrogen, phosphorus, potassium, calcium, magnesium and sodium in leaves shoots and roots of six olive cultivars. J. Plant Nutr., 25(11): 2457-2482.
  • LOUPASSAKI, M., LIONAKIS, S., ANDROULAKIS, I., 1997. Iron deficiency in kiwi and its correction by different methods. Acta Hort. 444: 267-271.
  • LOUPASSAKI, M., VASILAKAKIS, M., ANDROULAKIS, I., 1997. Effect of pre-incubation humidity and temperature treatment on the in vitro germination of avocado pollen grains. Euphytica 94: 247-251.
  • LOUPASSAKI, M., ANDROULAKIS, I., LIONAKIS, S., 1997. Effects of P and K fertilisers and the date of sampling on the concentration of macro and micro-elements in the leaves of four kiwi cultivars. Acta Hort. 444: 249-254.
  • ANDROULAKIS, I., LOUPASSAKI, M., SCHWABE, W., 1996. The content of mineral elements in the leaves of the olive cv. Koroneiki in relation to irrigation and the time of sampling. Acta Hort. 449: 119-124.
  • LOUPASSAKI, M., VASILAKAKIS, M., ANDROULAKIS, I., 1995. The time of flowering of avocado and the female and male opening of flowers in Crete. Adv. Hort. Sci. 9: 37-42.
  • LOUPASSAKI, M., PERICA, S., ANDROULAKIS, I., 1993. Seasonal changes in the olive fruit and the effects of Summer-applied nitrogen and potassium. Adv. Hort. Sci. 7: 65-68.



Dr Georgios Psarras
Assistant Researcher
Head of the Lab

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Tel: +30-28210-83440

Nektaria Digalaki

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Tel: +30-28210-83441

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